Isolation, identification and characterization of urinary tract infectious bacteria and the effect of different antibiotics

Abstract

Introduction: Urinary Tract Infection (UTI) defines a condition in which the urinary tract is infected with a pathogen causing inflammation which is a common, distressing and occasionally life threatening condition. UTI affects people of all ages and both gender. In all patients with UTI are reported with asymptomatic bacteriuria. Female are more susceptible to UTIs compared to male. To ensure appropriate therapy, current knowledge of the organisms that cause UTI and their antibiotic is susceptibility is mandatory. Methods: This study focused on the frequency of uropathogens and their antibiotic susceptibility in different gender in Madurai District. Cultural and biochemical characterization of uropathogens revealed the prevalence of both gram-positive and gram-negative organisms Results: E. coli was the predominant isolate isolated from the urine specimen followed by Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Proteus mirabilis and Enterococcus faecalis. Among the antibiotics tested, chloraphenicol and ciprofloxacin (100%) were found to be effective for empirical treatment of UTI and has covered the majority of urinary pathogens followed by tetracycline, gentamycin and kanamycin (83%), Ampicillin (67). Streptomycin, Rifampicin and amoxicillin were less effective (50%). Conclusion: Some of the isolates were resistant to penicillin-G, Streptomycin, rifampicin and amoxicillin which are more frequently prescribed and indicates that increased consumption of a particular antibiotic leads to acquisition of resistance by the uropathogens. Resistance rates among common uropathogens continue to evolve and appear to be increasing too many commonly used antimicrobial agents and a continued surveillance of resistance rates among uro