Association between Haematological parameters and Sickle cell genotypes in children with Plasmodium falciparum malaria residents in Kisumu County in Western Kenya

Abstract

ackground: Sickle cell disease (SCD) is a monogenic disorder due to point mutation in the β-globin gene resulting in substitution of Valine for Glutamic acid. The SCD is prevalent in P. falciparum endemic regions such as western Kenya. Carriage of different sickle cell genotypes may in uence haematological parameter during malaria. Children resident in malaria holoendemic regions suffer more from malariarelated complications and this is moderated by the presence of the SCD. In the current study, we determined the association between sickle cell genotypes and haematological parameters in children with P. falciparum malaria resident in Kisumu County in Western Kenya. Methodology: Children (n= 217, aged 1-192 months) with acute febrile condition were recruited at Jaramogi Oginga Odinga Teaching and Referral Hospital. Chi-square (c2) analysis was used to determine differences between proportions. Differences in haematological parameters were compared across groups using Kruskal Wallis test and between groups using Mann Whitney U test. Multivariate logistic regression analysis controlling for infection status was used to determine the association between sickle cell genotypes and haematological parameters. Results: Using HbAA as the reference group, multivariate logistic regression analysis revealed that carriage of HbSS was associated with reduced haemoglobin [OR= 0.310, 95% CI= 0.101-0.956, P= 0.041], reduced haematocrit [OR= 0.318, 95% CI= 0.128-0.793, P= 0.014], reduced RBC count [OR= 0.124, 95% CI= 0.045-0.337, P= 0.001], reduced MCHC [OR= 0.325, 95% CI= 0.118-0.892, P= 0.029], increased …